Description
The RefSeq mRNAs gene track for the 13 Aug 2020 Rhinolophus ferrumequinum/GCA_014108255.1_mRhiFer1.p
genome assembly displays translated blat alignments of vertebrate and
invertebrate mRNA in
GenBank.
Data Access
Download GCA_014108255.1_mRhiFer1.p.xenoRefGene.gtf.gz GTF file.
Track statistics summary
Total genome size: 2,072,561,339
Gene count: 18,531
Bases in genes: 862,603,053
Percent genome coverage: % 41.620
Methods
The mRNAs were aligned against the Rhinolophus ferrumequinum/GCA_014108255.1_mRhiFer1.p genome using
translated blat. When a single mRNA aligned in multiple places, the alignment
having the highest base identity was found. Only those alignments having a base
identity level within 1% of the best and at least 25% base identity with the
genomic sequence were kept.
Specifically, the translated blat command is:
blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl
where target.fa is one of the chromosome sequence of the genome assembly,
and the query.fa is the mRNAs from RefSeq
The resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25 -globalNearBest=0.0100 -minQSize=20
-ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap
all.results.psl GCA_014108255.1_mRhiFer1.p.xenoRefGene.psl
The filtered GCA_014108255.1_mRhiFer1.p.xenoRefGene.psl is converted to
genePred data to display for this track.
Credits
The mRNA track was produced at UCSC from mRNA sequence data
submitted to the international public sequence databases by
scientists worldwide.
References
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW.
GenBank.
Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42.
PMID: 23193287; PMC: PMC3531190
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.
GenBank: update.
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.
PMID: 14681350; PMC: PMC308779
Kent WJ.
BLAT - the BLAST-like alignment tool.
Genome Res. 2002 Apr;12(4):656-64.
PMID: 11932250; PMC: PMC187518
|