Description
The RefSeq mRNAs gene track for the 09 Jan 2023 Neoarius graeffei/GCF_027579695.1_fNeoGra1.pri
genome assembly displays translated blat alignments of vertebrate and
invertebrate mRNA in
GenBank.
Data Access
Download GCF_027579695.1_fNeoGra1.pri.xenoRefGene.gtf.gz GTF file.
Track statistics summary
Total genome size: 2,341,916,362
Gene count: 15,955
Bases in genes: 760,739,019
Percent genome coverage: % 32.484
Methods
The mRNAs were aligned against the Neoarius graeffei/GCF_027579695.1_fNeoGra1.pri genome using
translated blat. When a single mRNA aligned in multiple places, the alignment
having the highest base identity was found. Only those alignments having a base
identity level within 1% of the best and at least 25% base identity with the
genomic sequence were kept.
Specifically, the translated blat command is:
blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl
where target.fa is one of the chromosome sequence of the genome assembly,
and the query.fa is the mRNAs from RefSeq
The resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25 -globalNearBest=0.0100 -minQSize=20
-ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap
all.results.psl GCF_027579695.1_fNeoGra1.pri.xenoRefGene.psl
The filtered GCF_027579695.1_fNeoGra1.pri.xenoRefGene.psl is converted to
genePred data to display for this track.
Credits
The mRNA track was produced at UCSC from mRNA sequence data
submitted to the international public sequence databases by
scientists worldwide.
References
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW.
GenBank.
Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42.
PMID: 23193287; PMC: PMC3531190
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.
GenBank: update.
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.
PMID: 14681350; PMC: PMC308779
Kent WJ.
BLAT - the BLAST-like alignment tool.
Genome Res. 2002 Apr;12(4):656-64.
PMID: 11932250; PMC: PMC187518
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