Description
This track shows regions of the genome that are alignable
to other genomes ("chain" subtracks) or in synteny ("net" subtracks).
The alignable parts are shown with thick blocks that look like exons.
Non-alignable parts between these are shown like introns.
Chain Track
The chain track shows alignments of Mouse/(Jun. 2020 (GRCm39/mm39)) to the
lance-tailed manakin/Chiroxiphia lanceolata/GCF_009829145.1/2020-01-03 genome using a gap scoring system that allows longer gaps
than traditional affine gap scoring systems. It can also tolerate gaps in both
Mouse and lance-tailed manakin simultaneously. These
"double-sided" gaps can be caused by local inversions and
overlapping deletions in both species.
The chain track displays boxes joined together by either single or
double lines. The boxes represent aligning regions.
Single lines indicate gaps that are largely due to a deletion in the
Mouse assembly or an insertion in the lance-tailed manakin
assembly. Double lines represent more complex gaps that involve substantial
sequence in both species. This may result from inversions, overlapping
deletions, an abundance of local mutation, or an unsequenced gap in one
species. In cases where multiple chains align over a particular region of
the lance-tailed manakin genome, the chains with single-lined gaps are often
due to processed pseudogenes, while chains with double-lined gaps are more
often due to paralogs and unprocessed pseudogenes.
In the "pack" and "full" display
modes, the individual feature names indicate the chromosome, strand, and
location (in thousands) of the match for each matching alignment.
Net Track
The net track shows the best Mouse/lance-tailed manakin chain for
every part of the lance-tailed manakin genome. It is useful for
finding syntenic regions, possibly orthologs, and for studying genome
rearrangement. The Mouse sequence used in this annotation is from
the Jun. 2020 (GRCm39/mm39) assembly.
Display Conventions and Configuration
Chain Track
By default, the chains to chromosome-based assemblies are colored
based on which chromosome they map to in the aligning organism. To turn
off the coloring, check the "off" button next to: Color
track based on chromosome.
To display only the chains of one chromosome in the aligning
organism, enter the name of that chromosome (e.g. chr4) in box next to:
Filter by chromosome.
Net Track
At base level in full display mode, this track will show the
sequence of Mouse as it aligned to lance-tailed manakin. When the view is
too large to show such detail, a graph of the alignment score will be
shown.
Methods
Chain track
The Mouse genome was aligned to lance-tailed manakin genome with lastz.
The resulting alignments were converted into axt format using the lavToAxt
program. The axt alignments were fed into axtChain, which organizes all
alignments between a single Mouse chromosome and a single
lance-tailed manakin chromosome into a group and creates a kd-tree out
of the gapless subsections (blocks) of the alignments. A dynamic program
was then run over the kd-trees to find the maximally scoring chains of these
blocks.
Net track
Chains were derived from lastz alignments, using the methods
described on the chain tracks description pages, and sorted with the
highest-scoring chains in the genome ranked first. The program
chainNet was then used to place the chains one at a time, trimming them as
necessary to fit into sections not already covered by a higher-scoring chain.
During this process, a natural hierarchy emerged in which a chain that filled
a gap in a higher-scoring chain was placed underneath that chain. The program
netSyntenic was used to fill in information about the relationship between
higher- and lower-level chains, such as whether a lower-level
chain was syntenic or inverted relative to the higher-level chain.
The program netClass was then used to fill in how much of the gaps and chains
contained Ns (sequencing gaps) in one or both species and how much
was filled with transposons inserted before and after the two organisms
diverged.
The resulting net file was converted to axt format via netToAxt,
then converted to maf format via axtToMaf, then converted to
the bigMaf format with mafToBigMaf and bedToBigBed
Credits
lastz was developed by Robert Harris, Pennsylvania State University.
The axtChain program was developed at the University of California at
Santa Cruz by Jim Kent with advice from Webb Miller and David Haussler.
The browser display and database storage of the chains and nets were created
by Robert Baertsch and Jim Kent.
The chainNet, netSyntenic, and netClass programs
were developed at the University of California
Santa Cruz by Jim Kent.
References
Harris, R.S.
(2007) Improved pairwise alignment of genomic DNA
Ph.D. Thesis, The Pennsylvania State University
Chiaromonte F, Yap VB, Miller W.
Scoring pairwise genomic sequence alignments.
Pac Symp Biocomput. 2002:115-26.
PMID: 11928468
Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D.
Evolution's cauldron:
duplication, deletion, and rearrangement in the mouse and human genomes.
Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9.
PMID: 14500911; PMC: PMC208784
Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison RC,
Haussler D, Miller W.
Human-mouse alignments with BLASTZ.
Genome Res. 2003 Jan;13(1):103-7.
PMID: 12529312; PMC: PMC430961
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